Prevalence, risk factors and treatment outcomes of syphilis among people living with human immunodeficiency virus at primary care clinics in Malaysia: A retrospective study

Abstract Introduction: Syphilis and human immunodeficiency virus (HIV) coinfection is a common clinical problem with a significant rising trend worldwide. In Malaysia, the burden of care is shared between hospitals and primary care clinics. This study aimed to determine the prevalence of syphilis among people living with HIV (PLHIV) at primary care clinics in Malaysia and assess its association with sociodemographic characteristics, risk factors and treatment outcomes. Methods: This retrospective study included 750 PLHIV aged ≥18 years who attended primary care clinics in three different locations in Malaysia from 1 January 2017 to 31 December 2019. Data were obtained from the patients’ clinical notes using a structured questionnaire evaluating the sociodemographic characteristics, history of sexual and lifestyle behaviours, diagnosis and management. Results: The patients’ age ranged from 18 to 78 years (mean=34.7, standard deviation=10.2). The prevalence of syphilis among the PLHIV at the three primary care clinics was 33.8% (n=254). Syphilis was significantly associated with gender (P=0.038) as well as sexual activity (P<0.001), substance use (P=0.038), history of chemsex (P=0.001) and history of sexually transmitted infections (STIs) (P<0.001) within the past 12 months. The majority of the PLHIV with syphilis received treatment at the primary care clinics (n=248, 97.3%), and up to 96.1% (n=245) had completed such treatment. Conclusion: Syphilis is prevalent among PLHIV at primary care clinics, and most patients receive standard treatment. Therefore, primary care doctors must enhance their knowledge to effectively manage STIs, especially syphilis.


Introduction
Human immunode ciency virus (HIV) infection and syphilis are signi cant sexually transmitted infections (STIs) worldwide.e increasing incidence of syphilis at an early stage among people living with HIV (PLHIV) is worrying because it can facilitate the transmission of HIV and increase the incidence of new HIV infection later. 1 Coinfection with syphilis and HIV is predictable because of similar risk factors.Both diseases are synergistic in their transmission process, making the diagnosis and treatment more complex. 2prevalence of syphilis among PLHIV di ers across countries.In ailand, 16.1% of PLHIV are coinfected with syphilis and have higher HIV ribonucleic acid levels in their blood, seminal and rectal secretions than those with no syphilis.3 In Singapore, the incidence of syphilis increased 20 times from 2010 to 2017 and was consistently higher among men who have sex with men (MSM) than among heterosexual men. 4 A 5-year retrospective study on PLHIV with syphilis among MSM at several hospitals in Malaysia found that latent syphilis was the most common stage of diagnosis, and the failure rate was about 8.5% after a year of treatment.5 ere are several possible risk factors of syphilis, including insu cient immune defensive mechanism against HIV regardless of antiretroviral therapy (ART) status, risk compensation in sexual behaviour and susceptibility to Treponema pallidum infection.6 e recurrence of syphilis is associated with previous syphilis episodes, sexual risk behaviour, history of condomless anal sex with casual sexual partners and ART.7 e risk behaviours that contribute to infection among patients with STI and HIV in Malaysia include low condom use, increased number of sexual partners, history of STIs and history of sex with foreigners.8 is study aimed to assess the prevalence, risk factors and treatment outcomes of syphilis among MSM with HIV at selected STI primary care clinics in Malaysia.To date, there are no available published data on the prevalence, treatment and outcomes of syphilis among PLHIV at the primary care level in Malaysia.

Methods
is retrospective study included PLHIV who attended three primary care clinics in Malaysia, including the Mahmoodiah Primary Care Clinic in Johor Bahru and the Kuala Lumpur Primary Care Clinic and the Cheras Primary Care Clinic in Kuala Lumpur.Most newly diagnosed PLHIV on rst-line ART receive care at a public primary care clinic in Malaysia.e three clinics were selected based on the largest number of PLHIV in public primary care.e patients were selected based on the registration number from January 2017 to December 2019, and those who met the following inclusion criteria were included: age of ≥18 years, diagnosis of HIV, registration from 1 January 2017 to 31 December 2019 and maintenance of activity for at least a year after registration.e sample size of 750 participants was calculated using the Kish formula.e estimated prevalence of PLHIV in Malaysia was 115,263,9 indicating that a total of 662 participants were required.With a 99% con dence level, a 0.05 signi cance level and an 80% power, each clinic needed to collect 250 participants to achieve a total of 750 participants.e participants were randomly selected using standardised steps generated using an online randomiser and based on the registration number.
Data on sociodemographic characteristics and risk factors and management of syphilis were collected using data collection forms that were lled in manually by three investigators at the di erent study sites.
is study collected information on sociodemographic characteristics, risk factors, sexual history, treatment status, treatment outcome and noti cation and status of the partner(s) of patients diagnosed with syphilis.Syphilis was diagnosed based on laboratory ndings (positive rapid plasma regain (RPR) with a con rmatory Treponema pallidum partial agglutination (TPPA)).

Results
A total of 750 participants from each primary care clinic were included, with 250 participants randomly selected from each clinic.e mean age of the participants was 34.7±10.2years, and the largest proportion was aged 18-29 years.e sociodemographic characteristics and risk factors of the patients are shown in Table 1.
e majority of the PLHIV were homosexual (n=441, 58.8%), and up to 52.5% were sexually active within the past 12 months.Table 2 shows the diagnosis and stage of syphilis among the PLHIV.
e prevalence of syphilis among the PLHIV was 33.9% (n=254), and the most common stage of syphilis was late latent syphilis (68.5%, n=174).Table 3 shows the logistic regression analysis of the factors associated with syphilis among the PLHIV.In the simple logistic regression analysis, gender, marital status, educational level, employment status and sexual orientation as well as sexual activity, condom use, substance use, history of chemsex and history of STI within the past 12 months were found to be signi cant.e MLR analysis revealed that syphilis was signi cantly associated with gender, sexual activity, substance use, history of chemsex and history of STI.Table 4 shows the treatment status and outcome of the PLHIV with syphilis.e majority of the PLHIV with syphilis completed the treatment for syphilis, and most of them responded to such treatment.

Discussion
is study found a similar pattern of sociodemographic characteristics among the PLHIV relative to the latest prevalence of HIV transmission in Malaysia. 10e majority of the patients with syphilis were aged 18-29 years (n=109, 38.8%), while the minority were aged ≥60 years (n=1, 4.5%).Most patients were single (n=205, 80.4%), and a small number of patients were married/with partners (n=39, 15.3%).ese ndings are similar to previous reports on syphilis and HIV coinfection. 4,11 the patients in the present study, 85.1% received education at least at the secondary level, and only a small number had no formal education (n=2, 0.8%).ese data are di erent from those of a study conducted in Vietnam, where the total number of patients with syphilis who received primary education and no formal education was up to 19.5%. 12Although this study found that employment status was not an independent risk factor of syphilis, a study performed in Brazil suggested that employment is associated with a higher risk of syphilis among PLHIV based on the hypothesis that individuals with stable nances are more likely to engage in high-risk behaviours such as substance use and sex trade, including sex for money or sex for drugs. 13cause and association of syphilis with HIV infection can be related to sexual history, history of STI and lifestyle behaviours, all of which can serve as risk factors.In this study, MSM were the key population, as they made up 87.8% of the individuals positive for syphilis compared with only 10.6% of the heterosexuals.Although this study focused on the general population attending primary care clinics, the majority of those living with HIV in this study were MSM. is proportion may be comparable with that of other studies that have focused on MSM.4,5,7,11 Apart from sexual activity, the type of sexual practices and sexual partners are also important factors to explore as risk behaviours.Previous research has connected inconsistent condom use to the transmission of STIs including syphilis, making the advocation of consistently using condoms especially important to PLHIV who are sexually active.4 Substance use among the PLHIV in this study showed a slightly less di erence between those with and without syphilis.Conversely, a history of chemsex was more common among the PLHIV with syphilis than among those without syphilis.It is known that engaging in chemsex increases the risk of STI, such as having an increased number of partners, and reduces preventive measures, such as infrequent or lack of condom and pre-exposure prophylaxis use. 12 the present study, the MLR analysis revealed an association between syphilis and gender after the sociodemographic characteristics, sexual history and lifestyle behaviour were controlled.
4][15][16] Men are known to get involved with activities that can expose them to infection, such as having multiple sexual partners and sex trade. 16In this study, the odds ratio of the female patients developing syphilis was 10.5 times higher than that of the male patients, while the odds ratio of the married/with partner patients was 1.8 times higher than that of the single patients.Based on these data, both genders must be considered, and preventive measures must be emphasised for individuals in marital or partnered relationships.Other studies have supported the association of marital status with PLHIV attending specialised care service centres. 8,14While it may indicate an association, more focus needs to be given to the lifestyle behaviours of men who are married to reduce the risk of syphilis transmission.
In the MLR model in this study, the independent risk factors of syphilis among the PLHIV included sexual activity within the past 12 months, substance use in a lifetime and chemsex within the past 12 months.e noted association between chemsex and syphilis is similar to the report by DiDomizio within an Asian region. 17Conversely, Ahn et al. 18 reported that there was no indication of reduced syphilis risk with repeated episodes of syphilis.However, the authors considered that this association was limited in evidence because a history of syphilis may not re ect the current disease activity but rather the history of infection (based on the TPPA status).Accordingly, patients may remain positive for TPPA after treatment.However, the association between repeated episodes of syphilis and current active disease based on the TPPA status should be interpreted cautiously owing to the lack of direct testing in the MLR analysis in this study.e present study found that most PLHIV with syphilis completed the treatment for syphilis (n=245, 96.1%), and most patients responded to such treatment (n=166, 65.1%).
is is because most patients in primary care were newly diagnosed and received close monitoring for HIV infection and syphilis.A recent research conducted in a hospital setting in Malaysia revealed that up to 24.7% (n=42) of patients with syphilis were classi ed to have treatment failure. 19is nding is expected at a tertiary centre because most cases are referrals from a primary care centre, where cases can be complex and have been treated previously.Further, the immune system of patients with chronic and advanced stage of HIV infection may encounter challenges in eradicating syphilis. 20limitations of this study include the modest sample size and the cross-sectional design, which may not represent all PLHIV in Malaysia.Nonetheless, the ndings are still relevant considering that the majority of PLHIV are attending government outpatient clinics.Other limitations include the incomplete case records and lack of documentation.
Based on the present ndings, targeted interventions and preventive measures are recommended for PLHIV in Malaysia.Primary care doctors should educate all PLHIV regardless of gender and marital status on safe sexual practices and the dangers of chemsex to reduce syphilis transmission.

Conclusion
e prevalence of syphilis among the PLHIV at the three primary care clinics was fairly high (33.8%),with the majority being in the late stage of syphilis (68.5%).Gender, sexual activity, substance use, history of chemsex and history of STI were risk factors of syphilis.A large proportion of the patients (96.1%) completed the treatment for syphilis at the primary care clinics.

Open Access:
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Table 1 .
Sociodemographic characteristics and risk factors.

Table 2 .
Diagnosis and stage of syphilis.

Table 3 .
Simple and multiple logistic regression analyses of the factors associated with the risk of syphilis.

Table 3 . Continued
n: sample size, OR: odds ratio, P-value: probability value Basic assumptions that must be met for logistic regression include independence of error, linearity in the logit for continuous variables, absence of multicollinearity and lack of strong in uential outliers.No multicollinearity was found (VIF=1.085-1.830);Hosmer and Lemeshow test, P>0.05.

Table 4 .
Treatment status and outcome of the PLHIV with syphilis (n=255).
De nition: Responded to treatment: Four-fold drop in RPR levels at 1 year, Treated and defaulted: Defaulted after treatment, Reinfection: More than four-fold increase in RPR levels after 6 months of treatment, Failed treatment: No four-fold drop in RPR levels after 6 months and at the end of treatment.